The book is edited by Dr. Ganji, Division of Hematology and Oncology, University of Alabama and Dr. Venkatesan Amouda, Ampasala Dinakara Rao, Department of Bioinformatics, Pondicherry University. The book intends to decipher the knowledge in the emerging role of computational analysis for cancer therapy. Twenty chapters presented in this book focus on computational approaches that support the treatment by targeting abnormal metabolism in cancerous cells. With the advancement of technology, virtual screening has permitted the testing of a large number of new and repurposed chemicals in a short time at a much lower cost in a more efficient manner. It also provides a better understanding of artificial intelligence, drug repurposing, and targeting biomarkers in cancer treatment. This book would spark novel ideas and breakthrough research for the benefit of clinicians and patients who suffer from these fatal malignancies.